What quality control method do you use to make sure your total RNA is good? Do you run bioanalyzer or northern?
The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-Cl, pH 7.5). The RNA is treated by DNase I, and is tested as DNA free RNA by PCR. However, please be advised that total RNA from some tissues may not be treated by DNase I. Biochain is not using Agilent Bioanalyzer as a standard QC method for every total RNA products, though 80% of standard total RNA products are with Agilent Bioanalyzer data. If customers request RNAs without Agilent bioanalyzer data to betested on Agilent Bioanalyzer, additional fee will be applied. To visualize the RNA images on agarose gel, we recommend the same gel system be used as Biochain’s (1% agarose gel in 1xMOPS buffer with formaldehyde). Biochain is not responsible for customer getting degraded RNA images from other gel systems, such as TAE gel, TBE gel, Urea gel, etc.