I used your cDNA as template to amplify my target gene, but I can not get the signal, while I did get good signal of a house keep gene, what is the problem?
It is possible that the gene of your interest is a GC rich gene, optimizing your PCR condition by adding 0.5 M Betaine may help you solve the problem. Also, your target gene may be a low abundant gene, in such case, we would recommend you purchase mRNA either from BioChain or other sources, and use mRNA as template for cDNA synthesis.