The tissue lysates are run on 4-20% denaturing polyacrylamide gels and electrophoresed. After electrophoresis, the gel is transferred on to a PVDF membrane. This blot is ready to be probed with polyclonal, monoclonal, or peptide antibodies. The Protein marker is indicated at the left margin of the blot. The upper left corner of the membrane has been cut to provide orientation.